Differntial centrifugation is the process where a
homogenate (soup of tissue and cells) undergoes repeat
centrifugations and increasing centrifugal force. Centrifugations is
the use of increased gravity to quicken the precipitation of substances
tot the bottom. The tool used here is the centrifuge, "merry-go-round
for test tubes" that spin at various speeds.
The centrifuge separates the cell's parts into pellet and supernatant.
The pellet are the large cell structures that are settled at the test
tube's bottom. The supernatant are smaller parts of the cell suspending
in liquid, the supernatant is decanted and undergoes another
centrifugation. The process is repeated and increases speed with each
trial to collect successively smaller parts of a cell in pellets.
A. These are both test tubes attached to a
centrifuge. The first picture is of a test tube that has undergone
homogenization but is about to undergo centrifugation.The second
picture are the results of the centrifugation and portrays the settling
of large cell parts.
B. One of tools needed for differential
centrifugation is the centrifuge. This tool allows the cell components
to separate successively for the scientist. These are different types
of centrifuges but they both serve the same purpose.
C. This diagram shows the break down of the cell parts relating to the speed of the centrifuge.